The S-Phase Cyclin Clb5 Promotes rRNA Gene (rDNA) Stability by Maintaining Replication Initiation Efficiency in rDNA
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چکیده
منابع مشابه
TIF1 Represses rDNA replication initiation, but promotes normal S phase progression and chromosome transmission in Tetrahymena.
The non-ORC protein, TIF1, recognizes sequences in the Tetrahymena thermophila ribosomal DNA (rDNA) minichromosome that are required for origin activation. We show here that TIF1 represses rDNA origin firing, but is required for proper macronuclear S phase progression and division. TIF1 mutants exhibit an elongated macronuclear S phase and diminished rate of DNA replication. Despite this, repli...
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Approximately 700 nucleotide sequences surrounding the transcription initiation site were determined with a cloned rDNA fragment of Tetrahymena pyTiformis and the transcription initiation site was localized on these sequences using purified 35S pre-rRNA. A considerable portion of the 35S prerRNA was found to be capped in vitro. The P-labeled, capped 35S pre-rRNA, on nuclease PI protection mappi...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 2021
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.00324-20